Journal: Advanced Science

Article Title: Calpain‐2‐Mediated Endothelial Focal Adhesion Disruption in Thoracic Aortic Dissection

doi: 10.1002/advs.202501112

Figure Lengend Snippet: Conditional knockout of capns1 in endothelial cells prevented the formation of BAPN‐induced TAAD. A) En‐face immunofluorescence staining images depicting Calpain‐1, Calpain‐2, Capns1, and endothelial marker VE‐cadherin (VEC) in the descending aorta from Capns1 F/F and Capns1 △EC mice. Nuclei were counter‐stained using DAPI. Scale bar, 10 µm. B) The survival rate was estimated using the Kaplan‐Meier method and compared via the log‐rank test. C) The incidence of TAAD in Capns1 F/F and Capns1 △EC mice following 4 weeks of BAPN treatment. Saline‐Capns1 F/F , n = 6; Saline‐Capns1 △EC , n = 8; BAPN‐Capns1 F/F , n = 20; BAPN‐Capns1 △EC , n = 22. D) Quantification of the maximal diameters of both the ascending and descending aorta over the 4‐week period post‐BAPN treatment. Statistical significances were determined by two‐way ANOVA with Sidak's multiple comparision. E) Gross images depict the morphology of the entire aorta. Scale bar: 5 mm. F) The permeation of Evans blue dye into the thoracic aortas of Capns1 F/F and Capns1 △EC mice as assessed at 7 and 14 days after BAPN administration. G) Quantitative data on mRNA expression levels for Itgav, Vcl, and TIn1 ( n = 3 per group). Statistical significances were determined by two‐way ANOVA with Tukey's multiple comparision. H) Hematoxylin and eosin(HE), elastic van Gieson (EVG), and immunofluorescence (IF) staining of Itgav and Vinculin in cross‐sections of the Capns1 F/F and Capns1 △EC aorta.

Article Snippet: Antibodies for α‐SMA (rabbit IgG, 14395‐1‐AP), CD31 (rabbit IgG, 11265‐1‐AP), CD68 (rabbit IgG, 28058‐1‐AP), MMP9 (rabbit IgG, 10375‐2‐AP), Calpain‐2 (rabbit IgG, 11472‐1‐AP), Calpain‐1 (rabbit IgG, 10538‐1‐AP), VE‐cadherin (mouse IgG, 66804‐1‐Ig), Itgav (rabbit IgG, 27096‐1‐AP), Itgb3 (rabbit IgG, 18309‐1‐AP), Talin‐1 (rabbit IgG, 14168‐1‐AP), and Vinculin (mouse IgG, 66305‐1‐Ig) were sourced from Proteintech, China.

Techniques: Knock-Out, Immunofluorescence, Staining, Marker, Saline, Expressing

Journal: Advanced Science

Article Title: Calpain‐2‐Mediated Endothelial Focal Adhesion Disruption in Thoracic Aortic Dissection

doi: 10.1002/advs.202501112

Figure Lengend Snippet: The regulation of integrin activation and talin cleavage by Calpain‐2 in endothelial cells. Human aortic endothelial cells (HAECs) were transduced with Calpain‐2 via lentiviral transfection and subsequently treated with 1 µmol Angiotensin II (Ang II) for 24 h. A) The expression levels of Calpain‐2, Itgav, Itgb3, Talin, Vinculin, and VE‐cadherin were assessed using Western blotting. Representative Western blot images were presented, with GAPDH serving as the loading control. B) Quantitative data from the Western blot analysis ( n = 3 per group). C) Representative images of immunofluorescence staining for Itgav and VE‐cadherin in HAECs. Scale bar: 10 µm. D) Cell lysates from HAECs underwent co‐immunoprecipitation (Co‐IP) using anti‐Calpain‐2 or anti‐Itgav antibodies, followed by Western blotting. E) HA‐Capn2 or Flag‐Itgav were co‐transfected into HEK293T cells, demonstrating the colocalization of Calpain‐2 (green) and Itgav (red) in these cells. Scale bar:10 um. F) An adhesion assay was performed to evaluate the adhesion of THP‐1 monocytes to HAECs. Representative images showing DiI‐labeled THP‐1 monocyte adhesion to HAECs. Scale bar: 20 um. G) The relative number of THP‐1 monocytes adhering to HAECs following exposure to 1 µ m Ang II for 24 h was presented. Statistical significances were determined by One‐way ANOVA with Tukey's multiple comparision.

Article Snippet: Antibodies for α‐SMA (rabbit IgG, 14395‐1‐AP), CD31 (rabbit IgG, 11265‐1‐AP), CD68 (rabbit IgG, 28058‐1‐AP), MMP9 (rabbit IgG, 10375‐2‐AP), Calpain‐2 (rabbit IgG, 11472‐1‐AP), Calpain‐1 (rabbit IgG, 10538‐1‐AP), VE‐cadherin (mouse IgG, 66804‐1‐Ig), Itgav (rabbit IgG, 27096‐1‐AP), Itgb3 (rabbit IgG, 18309‐1‐AP), Talin‐1 (rabbit IgG, 14168‐1‐AP), and Vinculin (mouse IgG, 66305‐1‐Ig) were sourced from Proteintech, China.

Techniques: Activation Assay, Transduction, Transfection, Expressing, Western Blot, Control, Immunofluorescence, Staining, Immunoprecipitation, Co-Immunoprecipitation Assay, Cell Adhesion Assay, Labeling

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Expression of integrin αv, β3 in different ovarian tissues.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Immunohistochemical staining in ovarian malignant tumor ( A1 – A3 ), borderline tumor ( B1 – B3 ), benign tumor ( C1 – C3 ) and normal ovarian tissue ( D1 – D3 ). Integrin αv ( A1 , B1 , C1 , D1 ); β3 ( A2 , B2 , C2 , D2 ) and Lewis y ( A3 , B3 , C3 , D3 ). (Original magnification ×200).

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Immunohistochemical staining, Staining

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Relationship between integrin αv with the clinical and pathological parameters of malignant ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Diffusion-based Assay

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Relationship between integrin β3 with the clinical and pathological parameters of malignant ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Diffusion-based Assay

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Correlation between expression of Lewis y antigen and integrin αv in ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Correlation between expression of Lewis y antigen and integrin β3 in ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Integrin αv, β3 and Lewis y colocalize in ovarian malignant tumor. Using an immunofluorescent double-labeling method. Integrin αv and β3 ( A1 and B1 ); Lewis y ( A2 and B2 ); nucleus ( A3 and B3 ); Merged image ( A4 and B4 ). (Original magnification ×400).

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Labeling